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smad3 phosphorylation inhibitor  (TargetMol)


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    TargetMol smad3 phosphorylation inhibitor
    Smad3 Phosphorylation Inhibitor, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smad3+phosphorylation+inhibitor/pmc11145862-59-13-21?v=TargetMol
    Average 93 stars, based on 5 article reviews
    smad3 phosphorylation inhibitor - by Bioz Stars, 2026-07
    93/100 stars

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    ( A ) PCV2 infection induced Treg cell differentiation through the <t>TGF-β/Smad3</t> pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.
    Smad3 Phosphorylation Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smad3+phosphorylation+inhibitor/pmc12421831-40-3-17?v=MedChemExpress
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    smad3 phosphorylation inhibitor  (TargetMol)
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    TargetMol smad3 phosphorylation inhibitor
    ( A ) PCV2 infection induced Treg cell differentiation through the <t>TGF-β/Smad3</t> pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.
    Smad3 Phosphorylation Inhibitor, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smad3+phosphorylation+inhibitor/pmc11145862-59-13-21?v=TargetMol
    Average 93 stars, based on 1 article reviews
    smad3 phosphorylation inhibitor - by Bioz Stars, 2026-07
    93/100 stars
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    smad3 phosphorylation inhibitor sis3  (MedChemExpress)
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    ( A ) PCV2 infection induced Treg cell differentiation through the <t>TGF-β/Smad3</t> pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.
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    1 μm sis3 hcl (an inhibitor of smad3 phosphorylation)  (Sangon Biotech)
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    ( A ) PCV2 infection induced Treg cell differentiation through the <t>TGF-β/Smad3</t> pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.
    1 μm Sis3 Hcl (An Inhibitor Of Smad3 Phosphorylation), supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    smad3 phosphorylation  (TargetMol)
    93
    TargetMol smad3 phosphorylation
    ( A ) PCV2 infection induced Treg cell differentiation through the <t>TGF-β/Smad3</t> pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.
    Smad3 Phosphorylation, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smad3+phosphorylation+inhibitor/ppr0810069-58-5-9?v=TargetMol
    Average 93 stars, based on 1 article reviews
    smad3 phosphorylation - by Bioz Stars, 2026-07
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    ( A ) PCV2 infection induced Treg cell differentiation through the TGF-β/Smad3 pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.

    Journal: mBio

    Article Title: PCV2 infection induces the differentiation of Treg cells via the TGF-β/Smad3 pathway

    doi: 10.1128/mbio.01366-25

    Figure Lengend Snippet: ( A ) PCV2 infection induced Treg cell differentiation through the TGF-β/Smad3 pathway. Detection of the mRNA levels of IL-2 and TGF-β. PBMCs were isolated from peripheral blood at multiple time points post-challenge, and the mRNA expression levels were quantified by qPCR. ( B ) Detection of the expression levels of IL-2 and TGF-β. All animals were euthanized at 35 dpi, and cytokine levels were measured by ELISA. ( C ) The frequency of Treg cells. T cells were stimulated with various cytokines and analyzed for Treg cell frequency by flow cytometry. Unless otherwise specified, T cell stimulations lasted five days in vitro . ( D ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells. Foxp3 expression in Treg cells was quantified by calculating the arithmetic MFI using flow cytometry. ( E ) The subcellular localization of Smad3 in Treg cells. ( F ) Detection of phosphorylation level of Smad3. Total proteins were extracted from T cells and analyzed by Western blot for target protein expression. ( G ) pGL3-promoter-Foxp3 schematic diagram. ( H ) The double luciferase assay. The assay was used to investigate the effect of Smad3 on the activity of the Foxp3 enhancer. ( I ) Quantification of Foxp3 mRNA expression levels. ( J ) The frequency of Treg cells. ( K ) The MFI of Foxp3 in CD4 + Foxp3 + double-positive cells.

    Article Snippet: SIS3, a specific Smad3 phosphorylation inhibitor (HY-13013), and SB-431542, a TGF-β receptor inhibitor (HY-10431), were purchased from MedChemExpress.

    Techniques: Infection, Cell Differentiation, Isolation, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, In Vitro, Phospho-proteomics, Western Blot, Luciferase, Activity Assay

    The schematic diagram indicates that PCV2 infection induces the differentiation of Treg cells via the TGF-β/Smad3 pathway. PCV2 infection markedly enhances TGF-β secretion from host cells, subsequently activating the Smad3 signaling pathway in T cells. Phosphorylated Smad3 translocates into the nucleus, specifically binds to the Foxp3 gene enhancer region, and significantly increases Foxp3 transcription, ultimately promoting Treg cell differentiation. Notably, treatment with the Smad3-specific inhibitor SIS3 and TGF-β receptor inhibitor SB-431542 effectively inhibits Treg differentiation, confirming the crucial role of this pathway in PCV2-induced Treg cell differentiation.

    Journal: mBio

    Article Title: PCV2 infection induces the differentiation of Treg cells via the TGF-β/Smad3 pathway

    doi: 10.1128/mbio.01366-25

    Figure Lengend Snippet: The schematic diagram indicates that PCV2 infection induces the differentiation of Treg cells via the TGF-β/Smad3 pathway. PCV2 infection markedly enhances TGF-β secretion from host cells, subsequently activating the Smad3 signaling pathway in T cells. Phosphorylated Smad3 translocates into the nucleus, specifically binds to the Foxp3 gene enhancer region, and significantly increases Foxp3 transcription, ultimately promoting Treg cell differentiation. Notably, treatment with the Smad3-specific inhibitor SIS3 and TGF-β receptor inhibitor SB-431542 effectively inhibits Treg differentiation, confirming the crucial role of this pathway in PCV2-induced Treg cell differentiation.

    Article Snippet: SIS3, a specific Smad3 phosphorylation inhibitor (HY-13013), and SB-431542, a TGF-β receptor inhibitor (HY-10431), were purchased from MedChemExpress.

    Techniques: Infection, Cell Differentiation